Correlation between oligomycin-sensitivity and ergosterol in a purified mitochondrial adenosine triphosphatase from Saccharomyces cerevisiae.

use of mutants of Saccharomyces cerevisiae that, while maintaining the aerobic growth characteristics of the parent wild-type, show a resistance to oligomycin (Avner & Griffiths, 1970; Griffiths et al., 1972). This resistance appears to be located in the ATPsynthesizing or -hydrolysing enzyme (ATPaset) of the inner mitochondrial membrane. As a less complex material than entire mitochondrial membranes is desirable for investigation of the difference in oligomycin-sensitivity and its possible cause, we have purified and compared an ATPase from wild-type and mutant yeast mitochondria.